ONCOmmunity

Role of Phosphodiesterase2A in Proliferation and Migration of Human Osteosarcoma Cells.

By: Taku Murata, Kasumi Shimizu, Kazuto Kurohara, Akira Tomeoku, Gaku Koizumi, Naoya Arai

Department of Oral and Maxillofacial Surgery, Department of Clinical Sciences, Medical Life Science, Mie University, Graduate School of Medicine, Mie, Japan muratat@clin.medic.mie-u.ac.jp.
2019-09-17; doi: 10.21873/anticanres.13812

Abstract

Background/aim

The prognosis of patients with osteosarcoma is poor; therefore, new treatment strategies are urgently needed. Phosphodiesterase 2 (PDE2) is one of the 11 families (PDE1-PDE11) of the phosphodiesterase superfamily that regulates the intracellular concentrations and effects of cAMP and cGMP. This in vitro study was performed to investigate the role of PDE2 in human oral osteosarcoma HOSM-1 cells.

Materials

PDE2 expression was measured by a cAMP-PDE assay and real-time-PCR. The effects of the PDE2-specific inhibitors, erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA), 8-bromo-cAMP, and 8-bromo-cGMP on cell proliferation and migration were assessed.

Results

PDE2 activity and PDE2A mRNA expression were detected in HOSM-1 cells. Cell proliferation was inhibited by EHNA and 8-bromo-cAMP but not by 8-bromo-cGMP. Cell migration was stimulated by EHNA and 8-bromo-cGMP, but it was inhibited by 8-bromo-cAMP.

Conclusion

Cell proliferation is regulated by PDE2-cAMP signaling and cell migration is regulated by PDE2-cGMP signaling in HOSM-1 cells.