ONCOmmunity

Prognostic Significance of TYRO3 Receptor Tyrosine Kinase Expression in Gastric Cancer.

By: Chihiro Uejima, Masaki Morimoto, Manabu Yamamoto, Kazushi Hara, Wataru Miyauchi, Ken Sugezawa, Yoichiro Tada, Akimitsu Tanio, Kyoichi Kihara, Tomoyuki Matsunaga, Naruo Tokuyasu, Teruhisa Sakamoto, Soichiro Honjo, Yoshihisa Umekita, Yoshiyuki Fujiwara

Department of Surgery, Division of Gastrointestinal and Pediatric Surgery, Faculty of Medicine, Tottori University, Yonago, Japan.
2020-07-17; doi: 10.21873/anticanres.14572

Abstract

Background

Despite improved treatment for gastric cancer (GC), the prognosis of advanced disease remains poor. Further investigation of the oncogenic sequence for GC is needed.

Materials

The expression of TYRO3 protein tyrosine kinase in five GC cell lines was confirmed using western blotting. TYRO3 knockdown in GC cells, and bromodeoxyuridine and Transwell assays were used to examine the functions of TYRO3 in tumor proliferation and invasion. Finally, TYRO3 expression in 138 patients who underwent curative gastric resection for advanced GC (Union for International Cancer Control stage II/III) was tested by immunohistochemistry, and the association between prognosis and TYRO3 expression was analyzed.

Results

TYRO3 was detected at various levels in all the tested GC cell lines. Deleting TYRO3 significantly suppressed proliferation and invasion. Immunohistochemistry revealed TYRO3 expression was an independent prognostic factor for overall survival in patients with GC.

Conclusion

TYRO3 appears to mediate tumor progression and predict prognosis of patients with GC.