Neural crest-like stem cell transcriptome analysis identifies LPAR1 in melanoma progression and therapy resistance.
By: Jianglan Liu, Vito W Rebecca, Andrew V Kossenkov, Thomas Connelly, Qin Liu, Alexis Gutierrez, Min Xiao, Ling Li, Gao Zhang, Anastasia Samarkina, Delaine Zayasbazan, Jie Zhang, Chaoran Cheng, Zhi Wei, Gretchen M Alicea, Mizuho Fukunaga-Kalabis, Clemens Krepler, Pedro Aza-Blanc, Chih-Cheng Yang, Bela Delvadia, Cynthia Tong, Ye Huang, Maya Delvadia, Alice Santana Morais, Katrin Sproesser, Patricia Brafford, Joshua X Wang, Marilda Beqiri, Rajasekharan Somasundaram, Adina Vultur, Denitsa M Hristova, Lawrence W Wu, Yiling Lu, Gordon B Mills, Wei Xu, Giorgos C Karakousis, Xiaowei Xu, Lynn M Schuchter, Tara C Mitchell, Ravi K Amaravadi, Lawrence N Kwong, Dennie T Frederick, Genevieve M Boland, Joseph M Salvino, David W Speicher, Keith T Flaherty, Ze'ev A Ronai, Meenhard Herlyn

melanoma Research Center, The Wistar Institute.
2021-08-26; doi: 10.1158/0008-5472.CAN-20-1496
Abstract

Metastatic melanoma is challenging to clinically address. Although standard of care targeted therapy has high response rates in patients with BRAF-mutant melanoma, therapy relapse occurs in most cases. Intrinsically resistant melanoma cells drive therapy resistance and display molecular and biologic properties akin to neural crest-like stem cells (NCLSCs) including high invasiveness, plasticity and self-renewal capacity. The shared transcriptional programs and vulnerabilities between NCLSCs and cancer cells remains poorly understood. Here, we identify a developmental LPAR1-axis critical for NCLSC viability and melanoma cell survival. LPAR1 activity increased during progression and following acquisition of therapeutic resistance. Notably, genetic inhibition of LPAR1 potentiated BRAFi +/- MEKi efficacy and ablated melanoma migration and invasion. Our data defines LPAR1 as a new therapeutic target in melanoma and highlights the promise of dissecting stem cell-like pathways hijacked by tumor cells.





PMID:34462276






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