ONCOmmunity

Activity in MCF-7 Estrogen-sensitive Breast Cancer Cells of Capsicodendrin from Cinnamosma fragrans.

By: Ulyana Munoz Acuna, Nathan Ezzone, L Harinantenaina Rakotondraibe, Esperanza J Carcache DE Blanco

Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, Columbus, OH, U.S.A.
2021-10-08; doi: 10.21873/anticanres.15412

Abstract

Background/aim

Effect of capsicodendrin on the NF-κB pathway was studied in MCF-7 cancer cells.

Materials

The transcription factor assay was used to screen for NF-κB activity. The effect on IKKβ, ICAM-1, and caspase-7 were studied using western blot. Caspase-1 was studied using Promega Caspase-Glo^® assay. Reactive oxygen species (ROS) were detected using the fluorescent probe DCFH-DA. The potentiometric dye JC-1 was used to assess mitochondrial membrane potential (ΔΨm) and the cell cycle was examined using a fluorescence-activated cell sorter.

Results

NF-κB p65 inhibitory effect was IC₅₀=8.6 μM and cytotoxic activity was IC₅₀=7.5 μM. The upstream IKK and the downstream ICAM-1 were down-regulated. Sub G₁-phase population increased to 81% after 12 h of treatment with capsicodendrin (10 μM) and there was no loss of ΔΨM.

Conclusion

Increased levels of intracellular ROS promoted activity of caspase-1 and induced cell death in MCF-7 cells. Capsicodendrin may be a future anticancer agent that prevents the progression of metastatic breast cancer.