ONCOmmunity

Impact of the menstrual cycle on circulating cell-free DNA

By: Pölcher M, Ellinger J, Willems S, El-Maarri O, Höller T, Amann C, Wolfgarten M, Rudlowski C, Kuhn W, Braun M.

Department of Gynecology and Obstetrics, Center for Integrated Oncology, Bonn University Medical Center, Sigmund-Freud-Str. 25, D-53105 Bonn, Germany. martin.poelcher@ukb.uni-bonn.de
Anticancer Res. 2010 Jun; 30(6):2235-40.

Abstract

Background

To determine serum levels of circulating cell-free DNA (cfDNA) throughout the stages of endometrial proliferation and apoptosis during the menstrual cycle.

Materials and methods

cfDNA was measured in 176 blood samples from 17 healthy volunteers taken at three different time points (menstruation, follicular and secretory phase). Additionally, blood samples from 20 newly diagnosed breast cancer patients were analyzed. Quantitative real-time PCR was performed in order to quantify cfDNA fragments of 106 bp.

Results

In healthy individuals, levels of cfDNA did not differ significantly during the menstrual cycle. In breast cancer patients, the median cfDNA level was significantly higher compared to healthy individuals, irrespective of the cycle phase (p<0.001).

Conclusion

The female cycle does not influence cfDNA serum level measurements. Considering the diagnostic or prognostic value of cfDNA in cancer patients, different time points of blood sampling in premenopausal women seem to be negligible.

PMID: 20651374 [PubMed - indexed for MEDLINE] Source: National Library of Medicine.