Apoptosis induced by yessotoxins in Hela human cervical cancer cells in vitro
By: Pang M, Gao CL, Wu ZX, Lv N, Wang ZL, Tang XX, Qu P.
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P.R. China. pm_cat@hotmail.com.
Mol Med Report. 2010 Jul-Aug; 3(4):629-34. doi: 10.3892/mmr_00000307.
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P.R. China. pm_cat@hotmail.com.
Mol Med Report. 2010 Jul-Aug; 3(4):629-34. doi: 10.3892/mmr_00000307.
Abstract
To investigate the apoptotic effects of an extract of red-tide algae, Protoceratium reticulatum, on HeLa cells, we used liquid chromatography-tandem mass spectrometry, optical microscopy, Hoechst 33342/propidine iodide staining, and DNA gel electrophoresis to analyze its constituents and toxicity, as well as rhodamine 123 staining to investigate changes in mitochondrial membrane potential. Analysis showed that the P. reticulatum extract contained the yessotoxins (YTXs) homo-YTX, 45-OH-YTX and 45-OH-homo-YTX. The results indicated that P. reticulatum extract negatively influences HeLa cells and induces their apoptosis.
PMID: 21472289 [PubMed - in process] Source: National Library of Medicine.
