Melanoma is the most aggressive form of skin cancer, and the most rapidly expanding cancer in terms of worldwide incidence. Chemotherapeutic approaches to treat melanoma have been uniformly showing low efficiency. Previous studies in mice have demonstrated that a high diluted complex derivate from Calcarea carbonica (M8) stimulated the tumoricidal response of activated lymphocytes against B16F10 melanoma cells in vitro.

Here we described the in vitro inhibition of B16F10 cells invasion and the in vivo anti metastatic potential after M8 treatment by inhalation in the B16F10 lung metastasis model.

We found that M8 had at least two functions, as inhibitor of cancer cell adhesion and invasion and as perlecan expression antagonist, which have a strongly correlation with several metastatic, angiogenic and invasive factors in melanoma tumors.

The findings suggest that this medication is a promising complementary non−toxic therapy candidate, which may enhance the efficacy of conventional medicines by improving the immune response against tumor cells or even inducing direct dormancy in malignances.

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