The object of the present study was to identify markers for predicting urinary bladder cancer progression by comparative proteome analysis of bladder cancers and paired normal mucosas. We found that DDX39 was overexpressed in 4 of 6 bladder cancers examined compared with respective control tissues. Immunohistochemical analysis using 303 bladder cancer specimens revealed that DDX39 was inversely correlated to pT stage and histological grade progression. The incidences of DDX39 (high) tumors (positive cells ≥ 50%) were 68.6%, 43.5%, 20.0%, and 5.3% in pTa, pT1, pTis and ≥ pT2 tumors, and 65.2%, 60.7% and 19.6%　in G1, G2 and G3 tumors, respectively. The incidences of DDX39 (high) tumors were significantly lower in pT1 and ≥ pT2 compared to pTa tumors, and also significantly lower in G3 compared to G1 and G2 tumors. Follow-up analysis (n=105) revealed that patients with DDX39 (low) tumors (positive cells < 50%) were associated with disease progression (HR7.485, p=0.0083). Furthermore, DDX39-knockdown bladder cancer cells increased their ability for invasion compared to negative control cells. These results suggest that DDX39 is a suppressor of invasion and loss of its function predicts disease progression in bladder cancers.

© 2012 Japanese Cancer Association.

PMID: 22494014 [PubMed - as supplied by publisher] Source: National Library of Medicine.