ONCOmmunity

MicroRNA-19a and -19b regulate cervical carcinoma cell proliferation and invasion by targeting CUL5

By: Xu XM, Wang XB, Chen MM, Liu T, Li YX, Jia WH, Liu M, Li X, Tang H.

Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, China.
Cancer Lett. 2012 Sep 28; 322(2):148-58. Epub 2012 May 2.

Abstract

MicroRNAs (miRNAs) play a key role in the regulation of gene expression. In this study, we demonstrate that microRNA-19a and -19b (miR-19a/b) are highly expressed in human cervical cancer cells and are involved in maintaining the malignant phenotypes of HeLa and C33A cells. Up-regulation of miR-19a and miR-19b promoted cell growth and invasion, whereas knockdown of miR-19a and miR-19b yielded the reverse phenotype. CUL5 was identified as a novel target gene of both miR-19a and miR-19b. CUL5 ectopic over-expression without its 3' untranslated region (UTR) abolished the effect of miR-19a/b on HeLa and C33A cell proliferation and invasion. These results indicated that miR-19a/b directly and negatively regulate CUL5 expression in cervical cancer cells, highlighting the importance of miR-19a and miR-19b and their target genes in tumorigenesis.

PMID: 22561557 [PubMed - indexed for MEDLINE] Source: National Library of Medicine.