Histone acetyltransferase PCAF Up-regulated cell apoptosis in hepatocellular carcinoma via acetylating histone H4 and inactivating AKT signaling
Xin Zheng, Xiaohong Gai, Feihu Ding, Zhongtang Lu, Kangsheng Tu, Yingmin Yao and Qingguang Liu
Abstract
Background

PCAF is an important intrinsic histone acetyltransferases. This study tried to establish the effect of PCAF on HCC cell apoptosis.

Method

Both in vitro and in vivo experiments including IHC, DAPI staining, caspase3/7 activity assay, BrdU assay, MTT assay, western immunoblotting and co-immunoprecipitation were used here.

Results

PCAF was found to be expressed at the low level in most of HCC cell lines. PCAF overexpression induced cell apoptosis and growth arrest with increased Histone H4 acetylation and inactivation of AKT signaling in Huh7 and HepG2 cells. The opposite results were obtained by silencing PCAF in Hep3B cells. The co-immunoprecipitation assay confirmed that PCAF protein was bound with histone H4 protein in the nucleus of Hep3B cells. Finally, the in vivo experiment confirmed the findings mentioned-above.

Conclusions

These data identified PCAF promotes cell apoptosis and functions as a HCC repressor through acetylating histone H4 and inactivating AKT signaling.

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