Targeted Treatments: Pre-Clinical Evaluation Of Efficacy By Tracking Clonal Diversity In CLL Xenograft Models
Davies NJ, Oldreive CE, Agathanggelou A et al.
Key Points:
Multi-colour fluorescence in situ hybridization (MCF) to analyse CLL samples at single cell level to determine and follow clonal evolution in individual CLL cases.
Branched and linear pattern of evolution identified after analyzing 27 CLL samples.
Treatment has mixed effect on evolution.
Treatment effect was tested in in-vivo (mouse model) as well.
After rituximab therapy MCF showed that treatment affected all subclones with a differential subclonal response.
Implications:
- Differential response in different subclones important finding and novel therapies should be tested to see if capable of halting proliferation in cells with resistant phenotype or complex cytogenetics.
View the original abstract on the ASH website.
