Mapping The HLA Ligandome Of Chronic Lymphocytic Leukemia – Towards Peptide Based Immunotherapy
Kowalewski D, Schuster H, Berlin C et al.
Key Points:
Authors demonstrated new approach of direct isolation and identification of naturally processed and presented HLA ligands from tissues of interest.
ligands HLA class I isolated from MACS-sorted CLL cells and from normal B cells using standard purification protocol.
Authors able to map the HLA class I peptidomes of 25 CLL patients and 35 healthy controls, more than 25,000 different HLA ligands identified derived from CLL cells.
Implications:
- Identified vast array of novel proteins and demonstrated specific immune recognition of these peptides by CD8+ cells.
View the original abstract on the ASH website.
