The Co-Culture Of Primary CLL Cells
Purroy N, Abrisqueta P, Calpe E, et al.
Key Points:
- Investigators compared characteristics CLL cells found in PB and primary CLL cells co-cultured ex vivo in conditions mimicking microenvironment of proliferative niches (like bone marrow).
- Ki-67 positive CLL cells had higher expression levels of ZAP-70 & CD38 and lower levels of all chemokine receptors analyzed.
- Lethal dose 50 of fludarabine in co-culture of CLL cells not possible to calculate vs. 416μM (95%CI 125.5-1379)in CLL cells in suspension.
Implications:
- Study provides model for co-culture system which might serve as basis for development and testing of new drugs that target proliferative and drug resistant CLL cell compartment.
View the original abstract on the ASH website.
