ONCOmmunity

Vasohibin-1 increases the malignant potential of colorectal cancer and is a biomarker of poor prognosis.

By: Takahito Kitajima, Yuji Toiyama, Koji Tanaka, Susumu Saigusa, Minako Kobayashi, Yasuhiro Inoue, Yasuhiko Mohri, Masato Kusunoki

Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, Tsu, Mie, Japan.
2014-10-3; doi:

Abstract

Background

Vasohibin-1 (VASH1) is related to angiogenesis and poor prognosis in several types of cancers. However, the biological function and clinical significance of VASH1 in colorectal cancer (CRC) are not fully known.

Materials

The associations between VASH1 expression and clinicopathological features were investigated by immunohistochemistry in 429 CRC tissues. To evaluate the function of VASH1 in vitro, small-interfering VASH1-targeting RNA was transfected into human CRC cell lines.

Results

We found that VASH1 was highly expressed in the cytoplasm of CRC tissues. High VASH1 expression in the cytoplasm was significantly associated with tumor progression, such as larger tumor size, advanced T-stage, lymph node metastasis, distant metastasis and poor prognosis. Moreover, a significant positive correlation was detected between VASH1 expression and microvessel density. VASH1 siRNA inhibited CRC cell proliferation, migration, and invasion, and promoted anoikis.

Conclusion

Overexpression of VASH1 in CRC cells increased malignant potential and promoted metastasis.