ONCOmmunity

Lysophosphatidylserine Stimulates Chemotactic Migration of Colorectal Cancer Cells Through GPR34 and PI3K/Akt Pathway.

By: Yuuki Iida, Nelson H Tsuno, Junko Kishikawa, Kensuke Kaneko, Koji Murono, Kazushige Kawai, Toshiyuki Ikeda, Soichiro Ishihara, Hironori Yamaguchi, Eiji Sunami, Joji Kitayama, Yutaka Yatomi, Toshiaki Watanabe

Department of Surgical Oncology, Faculty of Medical Sciences, The University of Tokyo, Tokyo, Japan yiida-tky@umin.ac.jp.
2014-10-3; doi:

Abstract

Background

Lysophosphatidylserine (lysoPS) is a type of lysophospholipid mediator, which is involved in allergic conditions and tumor progression. We investigated the physiological function of lysoPS on colorectal cancer (CRC) cell lines, as well as the involved receptor and signaling pathways.

Materials

Expression of lysoPS receptors on six cell lines was examined by reverse transcription-polymerase chain reaction (RT-PCR). The physiological functions of lysoPS were investigated, and experiments using small interfering RNA (siRNA) or inhibitors of the signaling pathways were conducted.

Results

Among the three lysoPS receptors, GPR34 was highly expressed on all cell lines. LysoPS stimulated the chemotactic migratory ability. Wortmannin inhibited the migratory ability, as well as the GPR34 knock-down, strongly suggestive of the involvement of this receptor in the PI3K/Akt pathway.

Conclusion

The involved receptor and pathways in the migratory ability in response to lysoPS was demonstrated, which opens premises for targeting as a new strategy for prevention and treatment of colorectal cancer.