Survivin knock down by short hairpin RNA inhibits proliferation and induces apoptosis in EJ bladder cancer cells
By: Gou X, Yang HA, He WY, Xiao MC, Wang M.

The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Biochem Biophys Res Commun. 2010 May 8.

Abstract

Survivin is a 16.5 kDa member of the inhibitor of apoptosis protein family that is overexpressed in diverse malignancies including human bladder tumor, but not detectable in normal bladder tissues. We therefore investigated the effects of survivin gene RNA interference on cell proliferation and apoptosis of EJ human bladder cancer cells. We firstly constructed plasmids encoding short hairpin RNA (shRNA) targeting survivin which were then transfected into EJ cells using a cationic liposome based reagent. Survivin gene expression in EJ cells at the presence of survivin shRNA was assessed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis. Cell proliferation was determined by methyl thiazolyl tetrazolium assay. Cell cycle distribution and apoptosis were analyzed by flow cytometry. The effects of survivin shRNA on expression of caspase-3, Bax and Bcl-2 at mRNA levels were also investigated by RT-PCR. The survivin shRNA treatment caused a significant decrease in survivin expression at mRNA and protein levels. This inhibition of survivin expression was accompanied with an inhibition of cell proliferation in a time-dependent manner, G2/M accumulation, and an increase in the fraction of cells undergoing apoptosis. Survivin shRNA-induced apoptosis seemed to be Bax-dependent because survivin shRNA promoted Bax mRNA expression with no effect on caspase-3 or Bcl-2 expression. Our findings pave a way to target survivin to selectively inhibit cell proliferation of bladder cancer cells, and eventually for the treatment of bladder cancer. Copyright © 2010. Published by Elsevier Inc.

PMID: 20460110 [PubMed - as supplied by publisher] Source: National Library of Medicine.






* Albert Einstein College of Medicine has been
awarded Acceditation with Commendation by
the ACCME

Copyright 2025 InterMDnet | Privacy Policy | Disclaimer | System Requirements